Reconsideration of treatment protocol on the reduction of Enterococcus faecalis associated with failed root canal treatment

Woo Cheol Lee; Seong-Tae Hong; WonJun Shon

doi:10.5395/JKACD.2008.33.6.560

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Original Article Reconsideration of treatment protocol on the reduction of Enterococcus faecalis associated with failed root canal treatment: Woo Cheol Lee, Seong-Tae Hong, WonJun Shon; Journal of Korean Academy of Conservative Dentistry 2008;33(6):560-569.
DOI: https://doi.org/10.5395/JKACD.2008.33.6.560
Published online: November 30, 2008

Department of Conservative Dentistry, School of Dentistry, Seoul National University, Seoul, Korea.

Corresponding Author: Woo Cheol Lee. Department of Conservative Dentistry, School of Dentistry, Seoul National University & Dental Research Institute, 28 YunGun-Dong, JongNo-Gu, Seoul, Korea. 110-749, Tel: 82-2-2072-1634, Fax: 82-2-2072-3859, jimin525@snu.ac.kr

• Received: September 23, 2008 • Revised: October 21, 2008 • Accepted: October 23, 2008

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Abstract
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Abstract

Microorganism survived in the root canal after root canal cleaning and shaping procedure is a main cause of root canal treatment failure. There are several mechanisms for the bacteria to survive in the root canal after chemomechanical preparation and root canal irrigation. Bacteria organized as biofilm has been suggested as an etiology of persistent periapical lesion. Recent studies were focus on removal of Enterococcus faecalis biofilm due to the report that the persistence of this bacteria after root canal treatment may be associated with its ability to form biofilm. Several investigations demonstrated that current root canal treatment protocol including use of NaOCl, EDTA and Chlorhexidine as irrigants is quite effective in eliminating E. faecalis biofilm. However, this microorganism still can survive in inaccessible areas of root canal system and evade host immune response, suppress immune activity and produce biofilm. Up to date, there is no possible clinical method to completely get rid of bacteria from the root canal. Once the root canal treatment failure occurred, and conventional treatment incorporating current therapeutic protocol has failed, periapical surgery or extraction should be considered rather than prolong the ineffected retreatment procedure.
Keywords: Enterococcus faecalis; Treatment protocol; Biofilm; Persistent periapical lesion

REFERENCES

1. Imura N, Pinheiro ET, Gomes BPFA, Zaia AA, Ferraz CCR, Sauza-Filho FJ. The outcome of endodontic treatment : A retrospective study of 2000 cases performed by a specialist. J Endod. 2007;33: 1278-1282.Article PubMed
2. Swartz DB, Skidmore AE, Griffin JA Jr. Twenty years of endodontic success and failure. J Endod. 1983;9: 198-202.PubMed
3. Benenati FW, Khajotia SS. A Radiographic Recall Evaluation of 894 Endodontic Cases Treated in a Dental School Setting. J Endod. 2002;28: 391-395.PubMed
4. Gomes BP, Vianna ME, Sena NT, Zaia AA, Ferraz CCR, Souza-Filho FJ. In vitro evaluation of the antimicrobial activity of calcium hydroxide combined with chlorhexidine gel used as intracanal medicament. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;102: 544-550.PubMed
5. Siqueira JF, Araujo MCP, Garcia PF, Fraga RC, Dantas CJS. Histological evaluation of the effectiveness of five instrumentation techniques for cleaning the apical third of root canals. J Endod. 1997;23: 499-502.Article PubMed
6. Friedman S, Abitbol S, Lawrence HP. Treatment outcome in endodontics : The Toronto study. Phase 1: initial treatment. J Endod. 2003;29: 787-793.PubMed
7. Farzaneh M, Abitbol S, Lawrence HP, Friedman S. Treatment outcome in endodontics : The Toronto study. Phase II: initial treatment. J Endod. 2004;30: 302-309.PubMed
8. Marquis VL, Dao T, Farzaneh M, Abitbol S, Friedman S. Treatment outcome in endodontics : The Toronto study. Phase III: initial treatment. J Endod. 2006;32: 299-306.PubMed
9. de Chevigny C, Dao TT, Basrani BR, Marquis VL, Farzaneh M, Abitbol S, Friedman S. Treatment outcome in endodontics : The Toronto study. Phase 4: initial treatment. J Endod. 2008;34: 258-263.Article PubMed
10. Strindberg LZ. The dependence of the results of pulp therapy on certain factors. An analytic study based on radiographic and clinical follow-up examinations. Acta Odontol Scand. 1956;14: 1-175.
11. Peciuliene V, Balciuniene I, Eriksen HM, Haapasalo M. Isolation of Enterococcus faecalis in previously root-filled canals in a Lithuanian population. J Endod. 2000;26: 593-595.Article PubMed
12. Peciuliene V, Reynaud AH, Balciuniene I, Haapasalo M. Isolation of yeasts and enteric bacteria in root-filled teeth with chronic apical periodontitis. Int Endod J. 2001;34: 429-434.PubMed
13. Hancock HH, Sigurdsson A, Trope M, Moiseiwitsch J. Bacteria isolated after unsuccessful endodontic treatment in a North American population. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001;91: 579-586.Article PubMed
14. Pinheiro ET, Gomes BPFA, Ferraz CCR, Sousa ELR, Teixeira FB, Souza-Filho FJ. Microorganisms from canals of root-filled teeth with periapical lesions. Int Endod J. 2003;36: 1-11.Article PubMed PDF
15. Evans M, Davies K, Sundqvist G, Figdor D. Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide. Int Endod J. 2002;35: 221-228.Article PubMed
16. Sundqvist G, Figdor D, Persson S, Sjogren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998;85: 86-93.PubMed
17. Novais C, Vital C, Ribeiro G, Coque , Peixe LV. First characterization of vancomycin-resistant enterococci from a Portuguese hospital. J Antimicrob Chemother. 2002;49: 215-217.Article PubMed
18. Love RM. Enterococcus faecalis--a mechanism for its role in endodontic failure. Int Endod J. 2001;34: 399-405.Article PubMed PDF
19. Figdor D, Davies JK, Sundqvist G. Starvation survival, growth and recovery of Enterococcus faecalis in human serum. Oral Microbiol Immunol. 2003;18: 234-239.Article PubMed PDF
20. Rocas IN, Siqueira JF, Santos KR. Association of Enterococcus faecalis with different forms of periradicular diseases. J Endod. 2004;30: 315-320.PubMed
21. Gomes BP, Pinheiro ET, Sousa ELR, Jacinto RC, Zaia AA, Ferraz CCR, Souza-Filho FJ. Enterococcus faecalis in dental root canals detected by culture and by polymerase chain reaction analysis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;102: 247-253.Article PubMed
22. Jett BD, Huycke MM, Gilmore MS. Virulence of enterococci. Clin Microbiol Rev. 1994;7: 462-478.Article PubMed PMC PDF
23. Distel JW, Hatton JF, Gilespie MJ. Biofilm formation in medicated root canals. J Endod. 2002;28: 689-693.Article PubMed
24. Svensater G, Bergenholtz G. Biofilms in endodontic infections. Endod Topics. 2004;9: 27-36.
25. Duggan JM, Sedgley CM. Biofilm formation of oral endodontic Enterococcus faecalis. J Endod. 2007;33: 815-818.PubMed
26. Dunavant TR, Regan JD, Glickman GN, Solomon ES, Honeyman AL. Comparative evaluation of endodontic irrigants against Enterococcus faecalis biofilms. J Endod. 2006;32: 527-531.PubMed
27. Clegg MS, Vertucci FJ, Walker C, Belanger M, Britto LR. The effect of exposure to irrigant solutions on apical dentin biofilms in vitro. J Endod. 2006;32: 434-437.Article PubMed
28. Kakehashi S, Stanley HR, Fitzgerald RJ. The Effects of Surgical Exposures of Dental Pulps in Germ-Free and Conventional Laboratory Rats. Oral Surg Oral Med Oral Pathol. 1965;20: 340-349.PubMed
29. Moller AJ, Fabricius L, Dahlen G, Ohman AE, Heyden G. Influence on periapical tissues of indigenous oral bacteria and necrotic pulp tissue in monkeys. Scand J Dent Res. 1981;89: 475-484.Article PubMed
30. Sundqvist G. Bacteriologic studies of necrotic dental pulps. 1976;Umea University; Dissertation.
31. Sjogren U, Figdor D, Persson S, Sundqvist G. Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Int Endod J. 1997;30: 297-306.PubMed
32. Lin LM, Pascon EA, Skribner J, Gängler P, Langeland K. Clinical, radiographic, and histologic study of endodontic treatment failures. Oral Surg Oral Med Oral Pathol. 1991;71: 603-611.Article PubMed
33. Gomes BP, Pinheiro ET, Gade-Neto CR, Sousa ELR, Ferraz CCR, Zaia AA, Teixeira FB, Souza-Filho FJ. Microbiological examination of infected dental root canals. Oral Microbiol Immunol. 2004;19: 71-76.PubMed
34. Siqueira JF, Rocas IN. Polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004;97: 85-94.Article PubMed
35. Molander A, Reit C, Dahlén G, Kvist T. Microbiological status of root-filled teeth with apical periodontitis. Int Endod J. 1998;31: 1-7.Article PubMed
36. Ercan E, Ozekinci T, Atakul F, Gul K. Antibacterial activity of 2% chlorhexidine gluconate and 5.25% sodium hypochlorite in infected root canal: in vivo study. J Endod. 2004;30: 84-87.Article PubMed
37. Vianna ME, Horz HP, Gomes BP, Conreds G. In vivo evaluation of microbial reduction after chemomechanical preparation of human root canals containing necrotic pulp tissue. Int Endod J. 2006;39: 484-492.PubMed
38. Sassone LM, Fidel R, Fidel S, Vieira Hirata MR. The influence of organic load on the antimicrobial activity of different concentrations of NaOCl and chlorhexidine in vitro. Int Endod J. 2003;36: 848-852.Article PubMed PDF
39. Siqueira JF, Paiva SSM, Rocas IN. Reduction in the cultivable bacterial populations in infected root canals by a chlorhexidine-based antimicrobial protocol. J Endod. 2007;33: 541-547.Article PubMed
40. Ringel AM, Patterson SS, Newton CW, Miller CH, Mulhern JM. In vivo evaluation of chlorhexidine gluconate solution and sodium hypochlorite solution as root canal irrigants. J Endod. 1982;8: 200-204.Article PubMed
41. Shabahang S, Torabinejad M. Effect of MTAD on Enterococcus faecalis-contaminated root canals of extracted human teeth. J Endod. 2003;29: 576-579.PubMed
42. Torabinejad M, Cho Y, Khademi AA, Bakland LK. Shahrokh Shabahang The effect of various concentrations of sodium hypochlorite on the ability of MTAD to remove the smear layer. J Endod. 2003;29: 233-239.PubMed
43. Bystrom A, Claesson R, Sundqvist G. the antibacterial effect of camphorated para-monochlorophenol, camphorated phenol and calcium hydroxide in the treatment of infected root canals. Endod Dent Traumatol. 1985;1: 170-175.Article PubMed
44. Sjogren U, Figdor D, Spangberg L, Sundqvist G. The antimicrobial effect of calcium hydroxide as a short-term intracanal dressing. Int Endod J. 1991;24: 119-125.Article PubMed
45. Sathorn C, Parashos P, Messer H. Antibacterial efficacy of calcium hydroxide intracanal dressing: a systematic review and meta-analysis. Int Endod J. 2007;40: 2-4.PubMed
46. Haapasalo M, Orstavik D. In vitro infection and disinfection of dentinal tubules. J Dent Res. 1987;66: 1375-1379.Article PubMed PDF
47. Lin YH, Mickel AK, Chogle S. Effectiveness of selected materials against Enterococcus faecalis: part 3. The antibacterial effect of calcium hydroxide and chlorhexidine on Enterococcus faecalis. J Endod. 2003;29: 565-566.Article PubMed
48. McHugh CP, Zhang P, Michalek S, Eleazer PD. pH required to kill Enterococcus faecalis in vitro. J Endod. 2004;30: 218-219.Article PubMed
49. Tronstad L, Andreasen JO, Hasselgren G, Kristerson L, Riis I. pH changes in dental tissues after root canal filling with calcium hydroxide. J Endod. 1981;7: 17-21.PubMed
50. Haapasalo HK, Sirén EK, Waltimo TMT, Órstavik D, Haapasalo MPP. Inactivation of local root canal medicaments by dentine: an in vitro study. Int Endod J. 2000;33: 126-131.Article PubMed
51. Portenier I, Haapasalo H, Rye A, Waltimo T, Ørstavik D, Haapasalo M. Inactivation of root canal medicaments by dentine, hydroxylapatite and bovine serum albumin. Int Endod J. 2001;34: 184-188.Article PubMed PDF
52. Portenier I, Haapasalo H, Ørstavik D, Yamauchi M, Haapasalo M. Inactivation of the antibacterial activity of iodine potassium iodide and chlorhexidine digluconate against Enterococcus faecalis by dentin, dentin matrix, type-I collagen, and heat-killed microbial whole cells. J Endod. 2002;28: 634-637.Article PubMed
53. Sukawat C, Srisuwan T. A comparison of the antimicrobial efficacy of three calcium hydroxide formulations on human dentin infected with Enterococcus faecalis. J Endod. 2002;28: 102-104.PubMed
54. Cwikla SJ, Belanger M, Giguere S, Progulske-Fox A, Vertucci FJ. Dentinal tubule disinfection using three calcium hydroxide formulations. J Endod. 2005;31: 50-52.Article PubMed
55. Evans MD, Baumgartner JC, Khemaleelakul S, Xia T. Efficacy of calcium hydroxide: chlorhexidine paste as an intracanal medication in bovine dentin. J Endod. 2003;29: 338-339.Article PubMed
56. Gomes BP, Souza SF, Ferraz CC. Effectiveness of 2% chlorhexidine gel and calcium hydroxide against Enterococcus faecalis in bovine root dentine in vitro. Int Endod J. 2003;36: 267-275.Article PubMed PDF
57. Ercan E, Dalli M, Dulgergil CT. In vitro assessment of the effectiveness of chlorhexidine gel and calcium hydroxide paste with chlorhexidine against Enterococcus faecalis and Candida albicans. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;102: e27-e31.PubMed
58. Silva Garcez A, Núñez SC, Lage-Marques JL, Jorge AOC, Ribeiro MS. Efficiency of NaOCl and laser-assisted photosensitization on the reduction of Enterococcus faecalis in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;102: e93-e98.Article PubMed
59. Eldeniz AU, Ozer F, Hadimli HH, Erganis O. Bactericidal efficacy of Er,Cr:YSGG laser irradiation against Enterococcus faecalis compared with NaOCl irrigation: an ex vivo pilot study. Int Endod J. 2007;40: 112-119.Article PubMed
60. Bergmans L, Moisiadis P, Huybrechts B, Van Meerbeek B, Quirynen M, Lambrechts P. Effect of photo-activated disinfection on endodontic pathogens ex vivo. Int Endod J. 2008;41: 227-239.Article PubMed
61. Tronstad L, Barnett F, Cervone F. Periapical bacterial plaque in teeth refractory to endodontic treatment. Endod Dent Traumatol. 1990;6: 73-77.PubMed
62. Larsen T. Susceptibility of Porphyromonas gingivalis in biofilms to amoxicillin, doxycyclin and metronidazole. Oral Microbiol Immunol. 2002;17: 267-271.PubMed
63. Ramachandran Nair PN. Light and electron microscopic studies on root canal flora and periapical lesions. J Endod. 1987;13: 29-39.PubMed
64. Noiri Y, katsumoto T, Azakami H, Ebisu S. Effects of Er:YAG laser irradiation on biofilm-forming bacteria associated with endodontic pathogens in vitro. J Endod. 2008;34: 826-829.Article PubMed
65. George S, Kishen A, Song KP. The role of environmental changes on monospecies biofilm formation on root canal wall by Enterococcus faecalis. J Endod. 2005;31: 867-872.PubMed
66. Chai WL, Hamimah H, Cheng SC, Sallam AA, Abdullah M. Susceptibility of Enterococcus faecalis biofilm to antibiotics and calcium hydroxide. J Oral Sci. 2007;49: 161-166.Article PubMed
67. Lima KC, Fava LRG, Siqueira JF. Susceptibilities of Enterococcus faecalis biofilms to some antimicrobial medications. J Endod. 2001;27: 616-619.Article PubMed
68. Spratt DA, Pratten J, Wilson M, Gulabivala K. An in vitro evaluation of the antimicrobial efficacy of irrigants on biofilms of root canal isolates. Int Endod J. 2001;34: 300-307.Article PubMed PDF
69. Chavez de paz L. Redefining the persistent infection in root canals: possible role of biofilm communities. J Endod. 2007;33: 652-662.PubMed
70. Shenker BJ, McArthur WP, Tsai CC. Immune suppression Induced by Actinobacillus actinomycetemcomitans. I. Effects on human peripheral blood lymphocyte responses to mitogens and antigens. J Immunol. 1982;128: 148-154.Article PubMed PDF
71. Korostoff J, Wang JF, Kieba I, Miller M, Shenker BJ, Lally ET. Actinobacillus actinomycetemcomitans leukotoxin induces apoptosis in HL-60 cells. Infect Immun. 1998;66: 4474-4483.Article PubMed PMC PDF
72. Yoshida H, Jontell M, Sundqvist G, Bergenholtz G. Effect of sonicated material from Fusobacterium nucleatum on the functional capacity of accessory cells derived from dental pulp. Oral Microbiol Immunol. 1995;10: 208-212.Article PubMed
73. Shenker BJ, Datar S. Fusobacterium nucleatum inhibits human T-cell activation by arresting cells in the mid-G1 phase of the cell cycle. Infect Immun. 1995;63: 4830-4836.PubMed PMC
74. Lee W, Pankoski L, Zekavat A, Shenker BJ. Treponema denticola immunoinhibitory protein induces irreversible G1 arrest in activated human lymphocytes. Oral Microbiol Immunol. 2004;19: 144-149.Article PubMed
75. Lee W, Lim S, Son H, Bae K. Sonicated extract of Enterococcus faecalis induces irreversible cell cycle arrest in phytohemagglutinin-activated human lymphocytes. J Endod. 2004;30: 209-212.Article PubMed
76. Shon W, Lim S, Bae K, Baek S, Lee W. The expression of alpha4 integrins by human polymorphonuclear neutrophils in response to sonicated extracts of Enterococcus faecalis. J Endod. 2005;31: 369-372.PubMed
77. Giardino L, Ambu E, Savoldi E, Rimondini R, Cassanelli C, Debbia EA. Comparative evaluation of antimicrobial efficacy of sodium hypochlorite, MTAD, and tetraclean against Enterococcus faecalis biofilm. J Endod. 2007;33: 852-855.PubMed
78. Sena NT, Gomes BPFA, Vianna ME, Berber VB, Zaia AA, Ferraz CCR, Souza-Filho FJ. In vitro antimicrobial activity of sodium hypochlorite and chlorhexidine against selected single-species biofilms. Int Endod J. 2006;39: 878-885.Article PubMed

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Reconsideration of treatment protocol on the reduction of Enterococcus faecalis associated with failed root canal treatment

J Korean Acad Conserv Dent. 2008;33(6):560-569. Published online November 30, 2008

DOI: https://doi.org/10.5395/JKACD.2008.33.6.560

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Reconsideration of treatment protocol on the reduction of Enterococcus faecalis associated with failed root canal treatment

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