Matrix metalloproteinase-8 and substance P levels in root canal exudates of nonvital teeth

Article information

Restor Dent Endod. 2011;36(3):196-202

Publication date (electronic) : 2011 May 31

doi : https://doi.org/10.5395/JKACD.2011.36.3.196

Su-Jung Shin, DDS, MS ¹, Woocheol Lee, DDS, PhD ², Jae-Il Lee, DDS, MDS, PhD ³, Seung-Ho Baek, DDS, PhD ², Kee-Yeon Kum, DDS, PhD ², Won-Jun Shon, DDS, PhD ², Kwang-Shik Bae, DDS, MDS, PhD ²

¹Department of Conservative Dentistry, College of Dentistry, Yonsei University, Seoul, Korea.

²Department of Conservative Dentistry, Dental Research Institute, Seoul, Korea.

³Department of Oral Pathology, School of Dentistry, Seoul National University, Seoul, Korea.

Correspondence to Kwang-Shik Bae, DDS, MDS, PhD. Professor, Department of Conservative Dentistry, Seoul National University School of Dentistry and Dental Research Institute, 28-2 Yeongeon-dong, Jongro-gu, Seoul, Korea 110-749. TEL, +82-2-2072-2651; FAX, +82-2-764-3859; baeks@snu.ac.kr

Received 2011 March 10; Revised 2011 April 04; Accepted 2011 April 04.

Abstract

Objectives

The aim of this study was to investigate levels of matrix metalloproteinase-8 (MMP-8) and substance P (SP) in root canal exudates during root canal treatment (RCT) of nonvital, painful teeth.

Materials and Methods

Patients scheduled for nonsurgical RCT were prospectively selected; the study was performed after obtaining informed consent from the patients and was approved by the Institutional Review Board for Clinical Research of Gangnam Severance Hospital, Yonsei University (3-2008-0118). Canal exudates samples were collected using sterilized paper points from teeth scheduled for RCT across three different time periods. MMP-8 and SP levels were measured using enzyme-linked immunosorbent assay (ELISA). Data were analyzed using a mixed model analysis and the Pearson correlation analysis (p < 0.05).

Results

MMP-8 and SP levels in GCF were decreased during RCT (p < 0.0001), and they showed a weak positive correlation to each other (p < 0.05). Patients'subjective pain levels and the response from percussion test were significantly related to SP level.

Conclusions

This study demonstrated that periradicular inflammation endodontic origin can elevate SP and MMP-8 levels in root canal exudates. Interestingly, SP level of canal exudates showed a possibility of being used as an indicator of pain due to periapical pathosis.

Keywords: Canal exudate; MMP-8; SP; Pain

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Article information Continued

Figure 1

The amount of MMP-8 and SP from individual samples.

Figure 2

Corrected estimates and standard errors of MMP-8 and SP levels in the experimental group. A mixed model was used for adjusting patients' pain level.

Figure 3

Scatter plot of MMP-8 and SP at each period. (a) At first visit, (b) At second visit, (c) At third visit.