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The remineralization aspect of enamel according to change of the degree of saturation of the organic acid buffering solution in pH 5.5


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The remineralization aspect of enamel according to change of the degree of saturation of the organic acid buffering solution in pH 5.5

Article information

Restor Dent Endod. 2010;35(2):96-105
Publication date (electronic) : 2010 March 31
doi : https://doi.org/10.5395/JKACD.2010.35.2.096
Jin-Sung Park, Sung-Ho Park, Jeong-Won Park, Chan-Young Lee
Department of Dentistry, the Graduate School, Yonsei University, Seoul, Korea.
Corresponding Author: Chan-Young Lee. Department of Conservative Dentistry College of Dentistry, Yonsei University, 134 Shinchon-dong, Seodaemun-ku, Seoul, 120-752, Korea. Tel: 82-2-2228-8700, Fax: 82-2-313-7575, chanyoungl@yuhs.ac
Received 2009 December 28; Revised 2010 January 17; Accepted 2010 March 11.

Abstract

The purpose of this study is to observe and compare the remineralization tendencies of artificial enamel caries lesion by remineralization solutions of different degree of saturations at pH 5.5, using a polarizing microscope and computer programs (Photoshop, Image pro plus, Scion Image, Excel).

For this study, 48 sound permanent teeth with no signs of demineralization, cracks, or dental restorations were used. The specimens were immersed in lactic acid demineralization solution for 2 days in order to produce artificial dental caries that consist of surface and subsurface lesions. Each of 9 or 10 specimens was immersed in pH 5.5 lactic acid buffering remineralization solution of four different degrees of saturation (0.507, 0.394, 0.301, and 0.251) for 12 days. After the demineralization and remineralization, images were taken by a polarizing microscope (×100). The results were obtained by observing images of the specimens, and using computer programs, the density of caries lesions were estimated.

While the group with the lowest degree of saturation (0.251) showed total remineralization feature from the surface to the subsurface of the lesion, the group with the highest degree of saturation (0.507) showed demineralization mainly on the surface of the lesion at the constant organic acid concentration 0.01 M and pH 5.5.

Keywords: Demineralization; Remineralization; pH; Degree of saturation; Artificial cares; Enamel

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Article information Continued

Copyright © 2010 Korean Academy of Conservative Dentistry

Figure 1

Figure 1

Polarizing microscopic observation of demineralized enamel (Group 1, × 100).

Figure 2

Figure 2

Polarizing microscopic observation of remineralized enamel (Group 1, × 100).

Figure 3

Figure 3

Polarizing microscopic observation of demineralized enamel (Group 2, × 100).

Figure 4

Figure 4

Polarizing microscopic observation of remineralized enamel (Group 2, × 100).

Figure 5

Figure 5

Polarizing microscopic observation of demineralized enamel (Group 3, × 100).

Figure 6

Figure 6

Polarizing microscopic observation of remineralized enamel (Group 3, × 100).

Figure 7

Figure 7

Polarizing microscopic observation of demineralized enamel (Group 4, × 100).

Figure 8

Figure 8

Polarizing microscopic observation of remineralized enamel (Group 4, × 100).

Figure 9

Figure 9

Change ratio of demineralized depth

(demineralized depth after remineralization / demineralized depth before remineralization) × 100 (%).

Figure 10

Figure 10

Change rate of surface lesion width

(surface lesion width after remineralization / surface lesion width before remineralization) × 100 (%).

Figure 11

Figure 11

Comparison of density in enamel area before and after remineralization (Group 1).

Figure 12

Figure 12

Comparison of density in enamel area before and after remineralization (Group 2).

Figure 13

Figure 13

Comparison of density in enamel area before and after remineralization (Group 3).

Figure 14

Figure 14

Comparison of density in enamel area before and after remineralization (Group 4).

Figure 15

Figure 15

Change rate of remineralizalized amount before and after remineralization ((demineralized area before remineralization-demineralized area after remineralization/demineralized area before remineralization) ×100 + 100 (%)).

Table 1

Initial composition of demineralization solution

Table 1

Table 2

Initial composition of remineralization solution

Table 2

Table 3

One-way ANOVA on the remineralizing features of pH 5.5 solutions of different degree of saturations on artificially demineralized enamel

Table 3

* p < .05

Table 4

Demineralization depth (mm) and value change (%) during de- and remineralization

Table 4

Standard Deviation(SD) is in the parentheses.

Table 5

Surface lesion width (mm) and value change (%) during de-and remineralization

Table 5

Standard Deviation (SD) is in the parentheses.

Table 6

Remineralization amount (mm2) and value change (%) during de-and remineralization

Table 6

Standard Deviation (SD) is in the parentheses.