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Pulp response of beagle dog to direct pulp capping materials: Histological study


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Pulp response of beagle dog to direct pulp capping materials: Histological study

Article information

Restor Dent Endod. 2010;35(1):5-12
Publication date (electronic) : 2010 January 31
doi : https://doi.org/10.5395/JKACD.2010.35.1.005
Ji-Hyun Bae1, Young-Gyun Kim2, Pil-Young Yoon2, Byeong-Hoon Cho3orcid_icon, Yong-Hoon Choi1
1Department of Conservative Dentistry, Seoul National University Bundang Hospital, Korea.
2Department of Oral Maxillofacial Surgery, Seoul National University Bundang Hospital, Korea.
3Department of Conservative Dentistry, School of Dentistry, Seoul National University, Korea.
Corresponding Author: Yong-Hoon Choi. Department of Conservative Dentistry, Seoul National University Bundang Hospital, 300 Gumi-dong, Bundang-gu, Seongnam-si Gyeonggi-do, 463-707, Korea. Tel: 82-31-787-2780, Fax: 82-31-787-4068, yhchoi@snubh.org
Received 2009 September 08; Revised 2009 October 01; Accepted 2009 October 23.

Abstract

The purpose of this study was to evaluate the pulp tissue reaction to direct pulp capping of mechanically exposed beagle dogs'pulp with several capping materials. A total of 36 teeth of 2 healthy beagle dongs were used. The mechanically exposed pulps were capped with one of the followings: (1) Mineral Trioxide Aggregate (MTA: ProRoot® MTA, Dentsply, Tulsa, USA), (2) Clearfil SE Bond (Dentin adhesive system: Kuraray, Osaka, Japan), (3) Ultra-Blend (Photo-polymerized Calcium hydroxide: Ultradent, South Jordan, USA), (4) Dycal (Quick setting Calcium hydroxide: LD Caulk Co., Milford, USA) at 7, 30, and 90 days before sacrificing. The cavities were restored with Z350 flowable composite resin (3M ESPE, St. Paul. MN, USA). After the beagle dogs were sacrificed, the extracted teeth were fixed, decalcified, prepared for histological examination and stained with HE stain. The pulpal tissue responses to direct pulp capping materials were assessed.

In MTA, calcium hydroxide, and photo-polymerized calcium hydroxide groups, initial mild inflammatory cell infiltration, newly formed odontoblast-like cell layer and hard tissue bridge formation were observed. Compared with dentin adhesive system, these materials were biocompatible and good for pulp tissue regeneration.

In dentin adhesive system group, severe inflammatory cell infiltration, pulp tissue degeneration and pulp tissue necrosis were observed. It seemed evident that application of dentin adhesive system in direct pulp capping of beagle dog teeth cannot lead to acceptable repair of the pulp tissue with dentine bridge formation.

Keywords: Direct pulp capping; Pulp inflammation; Dentinal bridge; Histological evaluation; Beagle dog

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Article information Continued

Copyright © 2010 Korean Academy of Conservative Dentistry

Figure 1

Figure 1

a. Pulp capping with MTA at 7 days. Inflammatory cells infiltrated around the capping material under odontoblast-like cell. b. Pulp capping with MTA at 30 days. Newly formed dentinal bridge was observed. c. Pulp capping with MTA at 90 days. The thickness of hard tissue increased, compared with that of 30 days.

Figure 2

Figure 2

a. Pulp capping with SE Bond at 7 days. Inflammatory cells infiltrated accompanied by soft tissue disturbances. b. Pulp capping with SE Bond at 30 days. Coronal pulp tissue necrosis beneath the exposure site was observed. c. Pulp capping with SE Bond at 90 days. Severe pulp tissue necrosis beneath the exposure site was seen.

Figure 3

Figure 3

a. Pulp capping with Ultra-blend at 7 days. Inflammatory cell infiltration and hemorrhage was observed. b. Pulp capping with Ultra-blend at 30 days. Odontoblast-like cells arranged beneath exposure site. Pulp degeneration and partial pulp necrosis was seen. There was no hard tissue formation. c. Pulp capping with Ultra-blend at 90 days. Complete dentinal bridge protected the exposure site.

Figure 4

Figure 4

a. Pulp capping with Dycal at 7 days. Pulp tissue degeneration was seen. b. Pulp capping with Dycal at 30 days. Partial pulp tissue degeneration and hard tissue formation were observed. c. Pulp capping with Dycal at 90 days. Complete hard tissue formation and normal soft tissue organization beneath the exposure site.

Table 1

Criteria for grading inflammatory cell response

Table 1

Table 2

Criteria for grading hard tissue formation

Table 2

Table 3

Inflammatory responses and hard tissue formation resulted with 4 pulp capping materials

Table 3