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The effects of the fluoride concentration of acidulated buffer solutions on dentine remineralization


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The effects of the fluoride concentration of acidulated buffer solutions on dentine remineralization

Article information

Restor Dent Endod. 2009;34(6):526-536
Publication date (electronic) : 2009 November 30
doi : https://doi.org/10.5395/JKACD.2009.34.6.526
Won-Sub Han, Chan-Young Lee
Department of Conservative Dentistry, Yonsei University, Seoul, Korea.
Corresponding Author: Chan-Young Lee. Department of Conservative Dentistry, College of Dentistry, Yonsei University, 134, Sinchon-Dong, Seodaemun-Ku, Seoul 120-752, Korea. Tel: 82-2-2228-8700, chanyoungl@yuhs.ac
Received 2009 September 08; Revised 2009 October 23; Accepted 2009 October 27.

Abstract

The aim of this vitro-study is to evaluate the effects of fluoride on remineralization of artificial dentine caries. 10 sound permanent premolars, which were extracted for orthodontic reason within 1 week, were used for this study. Artificial dentine caries was created by using a partially saturated buffer solution for 2 days with grounded thin specimens and fractured whole-body specimens. Remineralization solutions with three different fluoride concentration (1 ppm, 2 ppm and 4 ppm) were used on demineralized-specimens for 7 days. Polarizing microscope and scanning electron microscope were used for the evaluation of the mineral distribution profile and morphology of crystallites of hydroxyapatite.

The results were as follows :

  1. When treated with the fluoride solutions, the demineralized dentine specimens showed remineralization of the upper part and demineralization of the lower part of the lesion body simultaneously.

  2. As the concentration of fluoride increased, the mineral precipitation in the caries dentine increased. The mineral precipitation mainly occurred in the surface layer in 1 and 2 ppm-specimens and in the whole lesion body in 4 ppm-specimens.

  3. When treated with the fluoride solution, the hydroxyapatite crystals grew. This crystal growth was even observed in the lower part of the lesion body which had shown the loss of mineral.

Keywords: remineralization; dentine; fluoride; Polarizing microscope; Scanning electron microscope

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Article information Continued

Copyright © 2009 The Korean Academy of Conservative Dentistry
(open-access) :

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Figure 1

Figure 1

Representative polarizing light microscopic images of 1 ppm specimen (×100).

(A-remineralization 1day, B-remineralization 5day, C-remineralization 7day)

Figure 2

Figure 2

Representative polarizing light microscopic images of 2 ppm specimen (×100).

(A-remineralization 1day, B-remineralization 5day, C-remineralization 7day)

Figure 3

Figure 3

Representative polarizing light microscopic images of 4 ppm specimen (×100).

(A-remineralization 1day, B-remineralization 5day, C-remineralization 7day)

Figure 4

Figure 4

Representative scanning electron microscopic (SEM) images of 1 ppm specimen (×10,000).

(A-10 µm, B-150 µm, C-200 µm, D-250 µm each from surface)

Figure 5

Figure 5

Representative SEM images of 2 ppm specimen ×10,000).

(A-50 µm, B-150 µm, C-200 µm, D-250 µm each from surface)

Figure 6

Figure 6

Representative SEM images of 4 ppm specimen (×10,000).

(A-50 µm, B-150 µm, C-200 µm, D-300 µm each from surface)

Figure 7

Figure 7

SEM images of superficial layer (×100,000).

(A-Normal dentine, B-Decalcified dentine, C-Dentine treated with 1 ppm re-mineralized solution for 7 days)

Figure 8

Figure 8

SEM images of upper part of lesion body (×100,000).

(A-Normal dentine, B,C and D-Dentine treated with 1,2 and 4 ppm re-mineralized solution each for 7 days)

Figure 9

Figure 9

SEM images of lower part of lesion body (×100,000).

(A-Normal dentine, B,C and D-Dentine treated with 1,2 and 4 ppm re-mineralized solution each for 7 days)

Table 1

Composition of decalcification solution.

Table 1

Table 2

Composition of remineralinzation solution.

Table 2