Comparison of viability of oral epithelial cells stored by different freezing methods
Article information
Restor Dent Endod. 2009;34(6):491-499
Publication date (electronic) : 2009 November 30
doi :
https://doi.org/10.5395/JKACD.2009.34.6.491
Received 2009 September 01; Revised 2009 September 25; Accepted 2009 October 06.
Abstract
This study examined the influence of the storage methods on the viability of oral epithelial cells using conventional cell freezing storage, slow freezing preservation, rapid freezing preservation, and slow freezing preservation with a pressure of 2 Mpa or 3 Mpa. The cell viability was evaluated by cell counting, WST-1 and the clonogenic capacity after 6 days of freezing storage. After 6 days, the frozen cells were thawed rapidly, and the cell counting, WST-1, and clonogenic capacity values were measured and compared.
The results from cell counting demonstrated that conventional cryopreservation, slow freezing under a 2 Mpa pressure and slow freezing under a 3 Mpa pressure showed significantly higher values than slow freezing preservation and rapid freezing preservation (p<0.05).
The results from the optical density by WST-1 demonstrated that slow freezing under a 2 Mpa pressure showed significantly higher values than slow freezing preservation and rapid freezing preservation (p<0.05).
The clonogenic capacity demonstrated that slow freezing under a 2 Mpa pressure showed significantly higher values than slow freezing preservation and rapid freezing preservation (p<0.05).
Keywords: WST-1; Cell counting; Clonogenic capacity; Slow freezing under pressure; Slow freezing; Rapid freezing
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