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EVALUATION OF <i xmlns="">ENTEROCOCCUS FAECALIS</i> REMOVAL EFFICACY OF THE ENDOVAC<sup xmlns="">®</sup> AND ENDOACTIVATOR<sup xmlns="">®</sup> INTRACANAL IRRIGATION METHODS

EVALUATION OF ENTEROCOCCUS FAECALIS REMOVAL EFFICACY OF THE ENDOVAC® AND ENDOACTIVATOR® INTRACANAL IRRIGATION METHODS

Article information

Restor Dent Endod. 2009;34(5):390-396
Publication date (electronic) : 2009 January 14
doi : https://doi.org/10.5395/JKACD.2009.34.5.390
Department of Conservative Dentistry, College of Dentistry, Gangneung-Wonju National University
*Corresponding Author: Jin-Woo Kim, Department of Conservative Dentistry, College of Dentistry, Gangneung-Wonju National University, 123 Chibyon-dong, Gangneung, Gangwon-do, Korea, Tel: 82-33-640-2470 Fax: 82-33-642-6410, E-mail: mendo7@kangnung.ac.kr
Received 2009 June 17; Revised 2009 July 20; Accepted 2009 September 04.

Abstract

Abstract

The aim of this study was to evaluate endodontic irrigation methods with EndoVac® and EndoActivator® in the elimination of Enterococcus faecalis from the root canals. Extracted 70 human single-rooted teeth were used. The canals were instrumented by a crown-down technique with .04 taper ProFile to ISO size 40. After the teeth were autoclaved, the canals were inoculated with E. faecalis and incubated for 48 h. The teeth were randomly divided into three experimental groups of 20 teeth each according to canal irrigation methods and two control groups as follows: group 1 - EndoVac®; group 2 - EndoActivator®; group 3 - Conventional needle irrigation method. After canal irrigation using 2.5% NaOCl, first samples (S1) were taken using sterile paper point. And the canals were filled with sterile brain heart infusion (BHI) broth and incubated for 24 h, then second samples (S2) were taken. The samples were cultured on BHI agar plate to determine the numbers of colony forming units (CFU). In first sampling (S1), only one canal of conventional method among the all experimental groups was positive cultured. In second sampling (S2), EndoVac® group showed the least positive culture numbers of E. faecalis. There was statistically significant difference between the EndoVac® and conventional needle irrigation methods in the mean value of Log CFU. According to the results of this study, EndoVac® showed better efficacy than conventional needle irrigation method in the elimination of E. faecalis from the root canal.

Numbers of Positive cultures

Mean values of the quantity of bacterial cells in secondary sampling (S2)

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Article information Continued

Table 1.

Numbers of Positive cultures

Irrigation method N Positive Culture
(S1) (S2)
EndoVac 20 0 (0%) 3 (15%)
EndoActivator 20 0 (0%) 8 (40%)
Conventional 20 1 (5%) 13 (65%)

Positive control group (n=5)

Negative control group (n=5)

Table 2.

Mean values of the quantity of bacterial cells in secondary sampling (S2)

Irrigation method Colony Forming Units /ml (S2)
  Mean (CFU/ml) Mean of LOG
EndoVac 0.99×105 0.87 *
EndoActivator 2.06×105 1.81
Conventional 4.12×105 2.90
*

There was significant(p < .05) difference between EndoVac and conventional irrigation method groups