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IN VITRO EVALUATION OF CLEANING EFFICACY OF VARIOUS IRRIGATION METHODS IN MANDIBULAR MOLARS

IN VITRO EVALUATION OF CLEANING EFFICACY OF VARIOUS IRRIGATION METHODS IN MANDIBULAR MOLARS

Article information

Restor Dent Endod. 2009;34(3):215-222
Publication date (electronic) : 2009 January 14
doi : https://doi.org/10.5395/JKACD.2009.34.3.215
Department of Conservative Dentistry, School of Dentistry, Seoul National University
*Corresponding Author: Seung-Ho Baek, Dept of Conservative Dentistry, School of Dentistry, Seoul National University, 28 Yeongon-dong, Jongno-gu, Seoul, Korea, Tel: 82-2-2072-3820 Fax: 82-2-747-0958 E-mail: shbaek@snu.ac.kr
Received 2009 April 06; Revised 2009 April 24; Accepted 2009 April 29.

Abstract

Abstract

The aim of this in vitro study was to evaluate the cleaning efficacy of various irrigation methods in the mandibular mesial roots. The forty five mesial root canals were shaped by Profile .06 instruments to apical size #30 and irrigated with 5 ml of 3.5% NaOCl. The teeth were divided into 3 groups and irrigated finally for 1 minute; Group 1: syringe irrigation, Group 2: ultrasonic irrigation, Group 3: RinsEndo irrigation.

After histological processing, the cross sections of apical 1, 3, and 5 mm level were examined with an optical microscope. The cleanliness values of canals and isthmuses were calculated and analyzed by Mann-Whitney U test.

  1. There were no significant differences in both canal and isthmus cleanliness between syringe irrigation and ultrasonic irrigation except 5 mm level of isthmus.

  2. RinsEndo irrigation had significantly higher canal cleanliness values than syringe irrigation at 1 mm and 3 mm levels (p < 0.05). Also, RinsEndo irrigation had significantly higher isthmus cleanliness values than syringe irrigation at all levels evaluated (p < 0.05).

  3. There were no statistical differences in both canal and isthmus cleanliness between ultrasonic irrigation and RinsEndo irrigation except 3 mm level of canal. From this study, RinsEndo irrigation can be useful as an additional irrigation procedure.

Figure 1.

Graph of mean percentage of canal cleanliness at 1, 3, and 5 mm levels

Figure 2.

Graph of mean percentage of isthmus cleanliness at 1, 3, and 5 mm levels

Figure 3.

Photomicrograph of cross section of the mesial root at the 5 mm level (group 1): 100% mesiobuccal canal (right) and 100% mesiolingual canal (left) cleanliness, with 41.1% isthmus cleanliness (40×, H&E stain)

Figure 4.

Photomicrograph of cross section of the mesial root at the 1 mm level (group 2): 100% mesiobuccal canal (right) and 100% mesiolingual canal (left) cleanliness, with 89.2% isthmus cleanliness (40×, H&E stain)

Figure 5.

Photomicrograph of cross section of the mesial root at the 1 mm level (group 3): 100% mesiobuccal canal (right) and 100% mesiolingual canal (left) cleanliness, with 100% isthmus cleanliness (40×, H&E stain)

Mean percentage of canal cleanliness at 1, 3, and 5 mm levels

Mean percentage of isthmus cleanliness at 1, 3, and 5 mm levels

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Article information Continued

Figure 1.

Graph of mean percentage of canal cleanliness at 1, 3, and 5 mm levels

* Statistically significant (p < 0.05).

Figure 2.

Graph of mean percentage of isthmus cleanliness at 1, 3, and 5 mm levels

* Statistically significant (p < 0.05).

Figure 3.

Photomicrograph of cross section of the mesial root at the 5 mm level (group 1): 100% mesiobuccal canal (right) and 100% mesiolingual canal (left) cleanliness, with 41.1% isthmus cleanliness (40×, H&E stain)

Figure 4.

Photomicrograph of cross section of the mesial root at the 1 mm level (group 2): 100% mesiobuccal canal (right) and 100% mesiolingual canal (left) cleanliness, with 89.2% isthmus cleanliness (40×, H&E stain)

Figure 5.

Photomicrograph of cross section of the mesial root at the 1 mm level (group 3): 100% mesiobuccal canal (right) and 100% mesiolingual canal (left) cleanliness, with 100% isthmus cleanliness (40×, H&E stain)

Table 1.

Mean percentage of canal cleanliness at 1, 3, and 5 mm levels

Level n Group 1 (%) n Group 2 (%) n Group 3 (%)
1 mm 25 91.7±12.7 29 93.5±10.2 21 97.3±9.9
3 mm 30 98.5± 3.0 30 98.4± 5.3 28 100.0±0.0
5 mm 30 99.7± 1.0 28 99.1±14.6 30 99.8±0.7

Table 2.

Mean percentage of isthmus cleanliness at 1, 3, and 5 mm levels

Level n Group 1 (%) n Group 2 (%) n Group 3 (%)
1 mm 8 46.3±35.6 10 59.0±30.5 8 82.5±26.4
3 mm 11 56.6±30.5 11 69.3±32.0 13 87.6±19.5
5 mm 10 51.0±33.2 15 85.4±29.6 13 88.1±21.5