Development of nano-fluid movement measuring device and its application to hydrodynamic analysis of dentinal fluid

Article information

Restor Dent Endod. 2008;33(2):141-147

Publication date (electronic) : 2008 March 31

doi : https://doi.org/10.5395/JKACD.2008.33.2.141

In-Bog Lee , Min-Ho Kim , Sun-Young Kim , Juhea Chang , Byung-Hoon Cho , Ho-Hyun Son , Seung-Ho Back

Department of Conservative Dentistry, School of Dentistry, Seoul National University, Korea.

Corresponding Author: In-Bog Lee. Department of Conservative Dentistry, School of Dentistry, Seoul National University, 275-1 Yeongeon-Dong, Jongno-Gu, Seoul 110-768, Korea. Tel: 82-2-2072-3953, Fax: 82-2-2072-3859, inboglee@snu.ac.kr

Received 2008 February 05; Revised 2008 March 03; Accepted 2008 March 08.

Abstract

This study was aimed to develop an instrument for real-time measurement of fluid conductance and to investigate the hydrodynamics of dentinal fluid. The instrument consisted of three parts; (1) a glass capillary and a photo sensor for detection of fluid movement, (2) a servo-motor, a lead screw and a ball nut for tracking of fluid movement, (3) a rotary encoder and software for data processing.

To observe the blocking effect of dentinal fluid movement, oxalate gel and self-etch adhesive agent were used. BisBlock (Bisco) and Clearfil SE Bond (Kuraray) were applied to the occlusal dentin surface of extracted human teeth. Using this new device, the fluid movement was measured and compared between before and after each agent was applied.

The instrument was able to measure dentinal fluid movement with a high resolution (0.196 nL) and the flow occurred with a rate of 0.84 to 15.2 nL/s before treatment. After BisBlock or Clearfil SE Bond was used, the fluid movement was decreased by 39.8 to 89.6%.

Keywords: Dentinal tubule; Water conductance; Dentin hypersensitivity; Oxalate; Self etching adhesive; Hydrodynamics

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This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Figure 1-a

Schematic diagram of the nano-flow measurement device.

Figure 1-b

Circuit diagram of photo sensor and servo amplifier.

Figure 2

Representative curves of dentinal fluid flow before and after application of BisBlock (a) or Clearfil SE bond (b).

Table 1

Water flow (nL) during 300 s and mean flow rate (nL/s) through dentin before and after application of BisBlock or Clearfil SE bond on the exposed dentin surface