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The effect of ethylene glycol analogs on mechanical properties of moist demineralized dentin matrix


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The effect of ethylene glycol analogs on mechanical properties of moist demineralized dentin matrix

Article information

Restor Dent Endod. 2006;31(4):290-299
Publication date (electronic) : 2006 July 31
doi : https://doi.org/10.5395/JKACD.2006.31.4.290
Kyung-Ha Lee, Young-Gon Cho1, Kwang-Won Lee
Department of Conservative Dentistry, School of Dentistry, Chonbuk National University, Korea.
1Department of Conservative Dentistry, College of Dentistry, Chosun University, Korea.
Corresponding Author: Kwang-Won Lee. Department of Conservative Dentistry, School of Dentistry, Chonbuk National University, 664-14 Duckjin-dong, Duckgin-gu, Jeonju, Korea, 561-756. Tel: +82-63-250-2016, lkw@chonbuk.ac.kr
Received 2006 April 04; Revised 2006 April 27; Accepted 2006 May 02.

Abstract

Objectives

The purpose of this study is to evaluate the effect of ethylene glycol analogs on modulus of elasticity and ultimate tensile strength of moist, demineralized dentin matrix.

Methods

Dentin disks 0.5 mm thick were prepared from mid-coronal dentin of extracted, unerupted, human third molars. "I" beam and hour-glass shaped specimens were prepared from the disks, the ends protected with nail varnish and the central regions completely demineralized in 0.5M EDTA for 5 days. Ultimate tensile stress (UTS) and low strain modulus of elasticity (E) were determined with specimens immersed for 60 min in distilled water (H2O), ethylene glycol (HO-CH2-CH2-OH), 2-methoxyethanol (H3CO-CH2-CH2-OH), and 1,2-dimethoxyethane (H3CO-CH2-CH3-OCH3) prior to testing in those same media. Modulus of elasticity was measured on the same specimens in a repeated measures experimental design. The results were analyzed with a one-way ANOVA on ranks, followed by Dunn's test at α = 0.05. Regression analysis examined the relationship between UTS or E and hoy's solubility parameter for hydrogen bonding (δh) of each solvent.

Results

The UTS of demineralized dentin in water, ethylene glycol, 2-methoxyethanol, and 1,2-dimethoxyethane was 24 (3), 30 (5), 37 (6), and 45 (6) MPa, × (SD) N = 10. Low strain E for the same media were 16 (13), 23 (14), 52 (24), and 62 (22) MPa. Regression analysis of UTS vs δh revealed a significant (p < 0.0001, r = -0.99, R2 = 0.98) inverse, exponential relationship. A similar inverse relationship was obtained between low strain E vs δh (p < 0.0005, r = -0.93, R2 = 0.86).

Significance

The tensile properties of demineralized dentin are dependent upon the hydrogen bonding ability of polar solvents (δh). Solvents with low δh values may permit new interpeptide H-bonding in collagen that increases its tensile properties. Solvents with high δh values prevent the development of these new interpeptide H-bonds.

Keywords: Polar Solvents; Ethylene Glycol Analogs; Hydrogen Bonding Ability

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Article information Continued

Copyright © 2006 Korean Academy of Conservative Dentistry
(open-access) :

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Figure 1

Figure 1

Schematic of sample preparation from mid-coronal dentin disk 0.5 mm thick (A). Hour-glass (B) and "I" beam (C) specimens were cut from the discs.

Figure 2

Figure 2

Two dimensional chemical structures of the control and test solvents.

Figure 3

Figure 3

Illustration of how the demineralized "I" beam was placed into friction grips of a universal testing machine.

Figure 4

Figure 4

Split-aluminum mold to measure the ultimate tensile strength by placing hourglass pattern into depressions.

Figure 5

Figure 5

UTS Values of Polar Solvents in 0.5 M EDTA Demineralized Dentin.

Figure 6

Figure 6

Hoy's Solubility Parameter vs UTS of Demineralized Dentin Matrix.

Figure 7

Figure 7

Hoy's Solubility Parameter vs UTS of Demineralized Dentin Matrix.

Figure 8

Figure 8

Low Wtrain Modulus of Elasticity of Polar Solvents.

Figure 9

Figure 9

Hoy's Solubility Parameter vs Low Strain Modulus of Elasticity.

Table 1

Hoy's solubility parameters, molecular weight and concentrations of test solvents

Table 1

hydrogen bonding forces (δh), dispersive forces (δd), polar forces (δp), Hildebrand's solubility parameter (δt). MV=molecular weight(g/mole)

Table 2

Solvent exposure order to the demineralized dentin

Table 2

Table 3

Summary of the effects of polar solvents on the tensile properties of demineralized dentin

Table 3

a UTS of specimens demineralized with 0.5 M EDTA.

b UTS of specimens demineralized with 37% H3PO4.

*5% strain modulus of elasticity.