Warning: mkdir(): Permission denied in /home/virtual/lib/view_data.php on line 81

Warning: fopen(upload/ip_log/ip_log_2024-12.txt): failed to open stream: No such file or directory in /home/virtual/lib/view_data.php on line 83

Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 84
A confocal microscopic study on dentinal infiltration of one-bottle adhesive systems and self-etching priming system bonded to class V cavities


Your browser does not support the NLM PubReader view.

Go to this page to see a list of supporting browsers.

A confocal microscopic study on dentinal infiltration of one-bottle adhesive systems and self-etching priming system bonded to class V cavities

Article information

Restor Dent Endod. 2002;27(3):257-269
Publication date (electronic) : 2002 May 31
doi : https://doi.org/10.5395/JKACD.2002.27.3.257
Hyung-Su Kim, Sung-Ho Park
Department of Dentistry, The Graduate School of Dentistry, Yonsei University, Korea.

Abstract

Objective

The purpose of this study was to evaluate the resin infiltration into dentin of one-bottle adhesive systems and self-etching primer bonded to Class V cavities using confocal laser scanning microscope(CLSM).

Material and Methods

Forty Class V cavities were prepared from freshly extracted caries-free human teeth. These teeth were divided into two groups based on the presence of cervical abrasion: Group I, cervical abrasion; Group II, wedge-shaped cavity preparation. Resin-dentin interfaces were produced with two one-bottle dentin bonding systems-ONE COAT BOND(OCB; Coltene®) and Syntac®Srint™(SS; VIVADENT)-, one self-etching priming system-CLEARFIL™ SE BOND(SB; KURARAY)- and one multi-step dentin bonding system-Scotchbond™Multi-Purpose(SBMP, 3M Dental Products)-as control according to manufacturers'instructions. Cavities were restored with Spectrum®(Dentsply). Specimens were immersed in saline for 24 hours and sectioned longitudinally with a low-speed diamond disc. The resin-dentin interfaces were microscopically observed using CLSM. The quality of resin-infiltrated dentin layers were evaluated by five dentists using 0-4 scale.

Results

Confocal laser scanning microscopal investigations using primer labeled with rhodamine B showed that the penetration of the primer occurred along the cavity margins.

Statistical analysis using one-way ANOVA followed by Duncan's Multiple Range test revealed that the primer penetration of the group 2(wedge-shaped cavity preparation) was more effective than group 1(cervical abrasion) and that of the gingival interfaces was more effective than the occlusal interfaces. In the one-bottle dentin bonding systems, the resin penetration score of OCB was compatible to SBMP, but those of SS and self-etching priming system, SB were lower than SBMP.

References

1. Pashley DH, Carvalho RM. Dentine permeability and dentine adhesion. J Dent 1997. 25355–372.
2. Ferrari M, Mannocci F, Cagidiaco MC, Kugel G. Short-term assessment of leakage of Class V composite restorations placed in vivo. Clin Oral Investig 1997. 1(2)61–64.
3. Gwinett AJ, Jendersen MD. Micromorphological features of cervical erosion after acid conditioning and its relation with composite resin. J Dent Res 1978. 57543–549.
4. Mixon JM, Spencer P, Moore DL, Chappell RP, Adams S. Surface morphology and chemical characterization of abrasion/erosion lesions. Am J Dent 1995. 85–9.
5. Heymann HO, Bayne SC. Current concepts in dentin bonding; focusing on dentinal adhesion factors. J Am Dent Assoc 1993. 12427–36.
6. Schupbach P, Krejci I, Lutz F. Dentin bonding: effect of tubule orientation on hybrid layer formation. Eur J Oral Sci 1997. 105344–352.
7. Van Meerbeek B, Braem M, Lambrechts P, Vanherle G. Morphological characterization of the interface between resin and sclerotic dentine. J Dent 1994. 22141–146.
8. Tay FR, Gwinnett AJ, Wei SH. Micromorphological spectrum from overdrying to overwetting acid conditioned dentin in water-free, acetone-based, single-bottle primer/adhesives. Dent Mater 1996. 236–244.
9. Watson TF, De Wilmot DM. A confocal microscopic evaluation of the interface between Syntac adhesive and tooth tissue. J Dent 1992. 20302–310.
10. Duke ES, Lindemuth J. Variability of clinical dentin sustrates. Am J Dent 1991. 4241–246.
11. Chappell RP, Cobb CM, Spencer P, Eick JD. Dentinal tubule anastomosis: a potential factor in adhesive bonding? J Prosthet Dent 1994. 72(2)183–188.
12. Ferrari M, Cagidiaco CM, Mason PN. Morphologic aspects of the resin-dentin interdiffusion zone with five different dentin adhesive systems tested in vivo. J Prosthet Dent 1994. 4. 71(4)404–408.
13. Duke ES. Clinical studies of adhesive systems. Oper Dent 1992. Suppl 5. 103–110.
14. Yoshiyama M, Carvalho R, Sano H, Horner JA, Brewer PD, Pashley DH. Regional bond strengths of resins to human root dentine. J Dent 1996. 435–442.
15. Hannig M, Reinhardt KJ, Bott B. Self-etching primer vs phosphoric acid:An alternative concept for composite-to-enamel bonding. Oper Dent 1999. 172–180.
16. Hannig M, Reinhardt KJ, Bott B. Self-etching primer vs phosphoric acid: An alternative concept for composite to enamel bonding. Oper Dent 1999. 24172–180.
17. Yoshiyama M, Sano H, Ebisu S, Tagami J, Ciucchi B, Carvalho RM, Johnson MH, Pashley DH. Regional strengths of bonding agents to cervical sclerotic root dentin. J Dent Res 1996. 751404–1413.
18. Watson TF. Application of confocal scanning optical microscopy to dentistry. Br Dent J 1991. 9287–291.
19. Minsky M. Microscopy apparatus 1957. Nov. 7. United States Patent Office Filed;
20. Yoshiyama M, Carvalho R, Sano H, Horner JA, Brewer PD, Pashley DH. Interfacial morphology and strength of bonds made to superficial versus deep dentin. Am J Dent 1995. 297–302.
21. Gwinnett AJ. Quantitative contribution of resin infiltration/hybridization to dentin bonding. Am J Dent 1993. 67–9.
22. Titley K, Chernecky R, Chan A, Smith D. The compositon and ultrastructure of resin tags in etched dentin. Am J Dent 1995. 8224–230.
23. Pashley DH, et al. Permeability of dentin to adhesive agents. Quintessence Int 1993. 24618–631.
24. Prati C, Chersoni S, Moniorgi R, Pashley DH. Resin-infiltrated dentin layer formation of new bonding systems. Oper Dent 1998. 23185–194.

Article information Continued

Copyright © 2002 Korean Academy of Conservative Dentistry
(open-access) :

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Fig. 1

Fig. 1

Diagram of longitudinally sectioned tooth showing cavity shapes (shadowed) and schematic orientation of dentinal tubules to the class V cavity surfaces.

Fig. 2

Fig. 2

Resin penetration scores in occlusal interfaces of class V restorations. Asterisk(*) means statistically significant differences(p<0.05)

Fig. 3

Fig. 3

Resin penetration scores in gingival interfaces of class V restorations. Asterisk(*) means statistically significant differences(p<0.05)

Fig. 4

Fig. 4

Comparison of resin penetrations of occlusal and gingival interfaces in group 1(cervical abrasion). Asterisk(*) means statistically significant differences (p<0.05)

Fig. 5

Fig. 5

Comparison of resin penetrations of occlusal and gingival interfaces in group 2(V-shaped cavity preparaion). Asterisk(*) means statistically significant differences(p<0.05)

Fig. 6.1-6.5

Fig. 6.1-6.5

Standard confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in occlusal interfaces of Class V cavity used for scoring the resin tag penetrations. Original magnification ×200

6.1. Score 0., 6.2. Score 1., 6.3. Score 2.,

6.4. Score 3., 6.5., Score 4.

Fig. 7.1-7.5

Fig. 7.1-7.5

Standard confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in gingival interfaces of Class V cavity used for scoring the resin tag penetrations. Original magnification ×200

7.1. Score 0., 7.2. Score 1., 7.3. Score 2.,

7.4. Score 3., 7.5., Score 4.

Fig. 8

Fig. 8

Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in gingival interfaces of Class V cavity with Scotchbond multi purpose. magnification × 800 23

Fig. 9.1-9.4

Fig. 9.1-9.4

Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in occlusal interfaces of cervical abrasion. Original magnification ×200

Fig. 10.1-10.4

Fig. 10.1-10.4

Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in occlusal interfaces of Class V cavity. Original magnification ×200

Fig. 11.1-11.4

Fig. 11.1-11.4

Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in gingival interfaces of cervical abrasion. Original magnification ×200

Fig. 12.1-12.4

Fig. 12.1-12.4

Confocal laser scanning microscopic(CLSM) images at resin-dentin interfaces in gingival interfaces of Class V cavity. Original magnification ×200

Table 1

Names, numbers and characteristics of each groups of experiment

Table 1

Table 2

Chemical components and instructions for use of the dentin bonding systems used in this study

Table 2

Table 3

Inter-observer correlations of each groups, materials, and parameters

Table 3

occlu: resin penetrations in occlusal interfaces.

gingi: resin penetrations in gingival interfaces.

Table 4

Resin penetration scores(Mean±SD) at the occlusal interfaces

Table 4

Comparison among the groups: one-way ANOVA, p<0.05

Table 5

Resin penetration scores(Mean±SD) at the gingival interfaces

Table 5