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Bacteriologic <i xmlns="">in vitro</i> coronal leakage study of before and after post space preparation


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Bacteriologic in vitro coronal leakage study of before and after post space preparation

Article information

Restor Dent Endod. 2005;30(1):16-21
Publication date (electronic) : 2005 January 31
doi : https://doi.org/10.5395/JKACD.2005.30.1.016
Hyo-An Lee, Eui-Seong Kim
Department of Conservative Dentistry, College of Dentistry, Yonsei University, Korea.
Corresponding author: Eui-Seong Kim. Department of Conservative Dentistry, College of Dentistry, Yonsei University, 134 Shinchon-Dong, Seodaemoon Gu, Seoul, 120-752, Korea. Tel: 82-2-361-8700, Fax: 82-2-313-7575, andyendo@yumc.yonsei.ac.kr
Received 2004 July 16; Revised 2004 November 12; Accepted 2004 November 23.

Abstract

The purpose of present study was to compare the speed of coronal leakage before and after post space preparation using Streptococcus mutans.

Forty straight extracted human teeth were selected. The crowns were removed to a uniform remaining root length 14 mm. Canals were enlarged by 06 taper Profiles® to a size #40 as a master apical file. And these were filled with gutta percha point and Tubuliseal® sealer, using continuous wave technique. Groupings are as follows.

Group 1 - These teeth were obturated without sealer.

Group 2 - These teeth were obturated and covered the surface of the root completely with sticky wax.

Group 3 - These teeth were obturated.

Group 4 - These teeth were obturated and prepared for post space remaining 5 mm of gutta percha.

The teeth were suspended in plastic tubes. The upper chamber received the bacterial suspension everyday to simulate clinical situation. The lower chamber consisted of BHI added Andrade's indicator.

All roots in the positive control group (Group 1) turned yellow within 24 h and those of negative control group (Group 2) remained red throughout the experimental period (70 days). The samples of group 3 were contaminated within an average of 27.2 days. The samples of group 4 were contaminated within an average of 15.7 days, ranging from 9 to 22 days.

There was significant difference between group 3 and group 4 statistically (p < 0.05).

Keywords: Coronal leakage; Post preparation; Bacteria

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Copyright © 2005 Korean Academy of Conservative Dentistry
(open-access) :

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Figure 1

Figure 1

Color change of model system at before(left) and after(right) coronal leakage

Figure 2

Figure 2

Comparison of time periods at which color change showed in lower chamber of model system