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Evaluation of microleakage with retrograde filling materials in blood contamination using fluid transport model


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Evaluation of microleakage with retrograde filling materials in blood contamination using fluid transport model

Article information

Restor Dent Endod. 2002;27(1):24-33
Publication date (electronic) : 2002 January 31
doi : https://doi.org/10.5395/JKACD.2002.27.1.024
Hyo-Soon Ahn, In-Ho Jang, Se-Joon Lee, Kwang-Won Lee
Department of Conservative Dentistry & Institute for Oral Bioscience, College of Dentistry, Chonbuk National University, Korea.

Abstract

Leakage studies have been performed frequently, since a fluid-tight seal provided by various dental filling materials has been considered clinically important. The leakage of the various root-end filling materials has been widely investigated mostly dye penetration method. These dye studies cannot offer any information about the quality of the seal of a test material over a long period of time The purpose of this study was to evaluate the microleakage of root end cavities in blood contamination filled amalgam, intermediate restorative material(IRM), light cured glass ionomer cement(GI) and mineral trioxide aggregate(MTA) by means of a modified fluid transport model. Fifty standard human root sections, each 5mm high and with a central pulp lumen of 3mm in diameter, were and filled with our commonly used or potential root end filling materials after they were contaminated with blood. At 24h, 72h, 1, 2, 4, 8, and 12 weeks after filling, leakage along these filling materials was determined under a low pressure of 10KPa(0.1atm) using a fluid transport model.

The results were as follows:

1. MTA group showed a tendency of decreasing percent of gross leakage (20ml/day) in process of time, whereas the other materials showed a tendency of increasing in the process time.

2. At the all time interval, GI group leaked significantly less than amalgam group and IRM group (p<0.05).

3. At the 4 weeks, the percentage of gross leakage in MTA group decreased to 0% thereafter, the low percentage of gross leakage was maintained in MTA group until the end of the experiment, whereas the percentage in IRM group increased to 100%.

4. At the 12 weeks, percentage of gross leakage was significantly low in MTA group(0%), comparison with GI group(40%), amalgam group(90%) and IRM group(100%), but there was no significant difference between latter two materials.

References

1. Sjögrem U, Hägglund B, Sundqvist G, Wing K. Factors affecting the long term results of endodontic treatment. J Endod 1990. 16498–504.
2. Ingle JI, Backland LK. Endodontics 1994. 4th edth ed. Philadelphia: Lea & Febiger Book; 689–763.
3. Baumgartner JC, Galkler WA. Bacteria in the apical 5mm of infected root canals. J Endod 1991. 17380–383.
4. Harty FJ, Parkins BJ, Wengrat AM. The success rate of Apicoectomy, A retrospective study of 1016 case. Br Dent J 1970. 129407–418.
5. Gartner A, Dorn S. Advances in endodontic surgery. Dent Clin North Am 1992. 36357–378.
6. Cho YB, Hong CU, Shin DH. Effects of some root end filling materials on the repair of periradicular tissue. J Korean Acad Conserv Dent 1995. 20(1)17–32.
7. Rud J, Andreason JO, Jensen JEM. Radiographic criteria for the assessment of healing after endodontic surgery. Int J Oral Surg 1972. 1195–214.
8. Dorn SO, Gartner A. Retrofilling materials : a retrospective success-failure study of amalgam, EBA, and IRM. J Endod 1990. 16391–393.
9. Frank AL, Click DH, Paterson SS, Weine FS. Long-term evaluation of surgically placed amalgam fillings. J Endod 1992. 18391–398.
10. Rapp EL, Grown CE, Newton CW. An analysis of success and failure of apicoectomies. J Endod 1991. 17508–512.
11. Smee G, Bolanos OR, Morse DR, Furst MF, Yesilsoy C. A comparative leakage study of P-30 resin bonded ceramic, Teflon, amalgam, and IRM as retrofilling seals. J Endod 1987. 13117–121.
12. Bondra DL, Hartwell GR, Macpherson MG, Portell FR. Leakage in vitro with IRM, high copper amalgam, and EBA cement as retrofilling materials. J Endod 1989. 15157–160.
13. Wilson AD, Kent BE. A new translucent cement for dentistry. Br Dent J 1972. 132133–135.
14. Tobias RS, Brows RM, Plant CG, INgram DW. Pulpal response to a glass ionomer cement. Br Dent J 1978. 144345–350.
15. Pitt Ford TR, Robers GJ. Tissue response to glass ionomer retrograde root fillings. Int Endod J 1990. 23233–238.
16. Abdal Ak, Retief DH. The apical seal via the retrosurgical approach 1 A Preliminary study. Oral Surg 1982. 53614–621.
17. Ruyter IE, Sjøvik IJ. Monomer composition of dental composites and sealants. J Dent Res 1978. 57(spec. iss)249. abstr. 700.
18. Rueggeberg FA, Craig RG. Correlation of parameters used to estimate monomer conversion in a light-cured composite. J Dent Res 1988. 67932–937.
19. Asmussen E. Restorative resins: hardness and strength vs quantity of remaining double bonds. Scand J Dent Res 1982. 90484–489.
20. Blackman R, Gross M, Seltzer S. An Evaluation of the biocompatibility of a glass ionomer-silver cement in rat connective tissue. J Endod 1989. 1576–79.
21. Pissiotis E, Sapounas G, Spangberg LSW. Silver glass ionomer cement as a retrograde filling material : a study in vitro. J Endod 1991. 17225–229.
22. Bates CF, Carnes DL, del Rio CE. Longitudinal sealing ability of mineral trioxide aggregate as a root-end filling material. J Endod 1996. 22(11)575–578.
23. Torabinejad M, Watson TF, Pitt Ford TR. Sealing ability of a mineral trioxide aggregate when used as a root end filling material. J Endod 1993. 19591–595.
24. Torabinejad M, Dby Wc, Naidorf IJ. Inflammatory and immunological aspects of the pathogenesis of human periapical lesions. J Endod 1985. 11479–488.
25. Torabinejad M, Hong CU, Pitt Ford TR, Kettering JD. Cytotoxiciy of four root end filling materials on mouse L929 cells. J Endod 1993. 19203.
26. Hong Cu, McKendry DJ, Pitt Ford TR, Torabinejad M. Healing of furcal lesions repaired by amalgam or mineral trioxide aggregate. J Endod 1994. 29197.
27. Torabinejad M, Hong CU, Kettering JD. Antibacterial effects of some root end filling materials. J Endod 1995. 21403–406.
28. Torabinejad M, Higa RK, McKendry DJ, Pitt Ford TR. Dye leakage of four root end filling materials: Effects of blood contamination. J Endod 1994. 20(4)159–163.
29. Pashley DH, Thompson SM, Stewart FP. Dentin permeability: effects of temperature on hydraulic conductance. J Dent Res 1983. 62956–959.
30. Wu MK, De Gee AJ, Wesselink PR. Leakage of four root canal sealers at different thicknesses. Int Endod J 1994. 27304–308.
31. Derkson GD, Pashley DH, Derkson ME. Microleakage measurement of selected restorative materials: a new in vitro method. J Prosthet Dent 1986. 56435–440.
32. King KT, Anderson RW, Pashley DH, Pantera EA. Longitudinal evaluation of the seal of endodontic retrofillings. J Endod 1990. 16307–310.
33. Trowbridge HO, Kim S. Pulp development, structure and function. Pathways of the pulp 1994. 6th edth ed. St. Louis: Mosby; 322–325.
34. Wu MK, Kontakiotis EG, Wesselink PR. Long-term seal provided by some root-end filling materials. J Endod 1998. 24(8)557–560.
35. Wu MK, Kontakiotis EG, Wesselink PR. Decoloration of 1% methylene blue solution in contact with dental filling materials. J Dent 1998. 26585–589.
36. Philips RW. Skinner's science of dental materials 1973. 7th edth ed. Philadelphia: WB b Saunders;
37. Callis PD, Mannan G. Microleakage of retrograde fillings: assessment using a novel measurement system. J Endod 1994. 20555–557.
38. Inose S, Yoshimura M, Tinkle JS, Marshall FJ. A 24-week study of the microleakage of four retrofilling materials using a fluid filtration method. J Endod 1991. 17369–375.
39. Kent BE, Lewis BG, Wilson AD. The properties of glass ionomer cement. Br Dent J 1973. 135322–326.
40. Barkhordar RA, Pelzner RB, Stark MM. Use of glass ionomers as retrofilling materials. Oral Surg Oral Med Oral Pathol 1989. 67734–739.
41. Roth S. A laboratory study of glass ionomer cement as a retrograde filling material. Aust Dent J 1991. 36(5)384–390.
42. Chong BS, Pitt Ford TR, Watson FF, Wilson RF. Sealing ability of potential retrograde root filling materials. Endod Dent Traumatol 1995. 11264–269.
43. Jesslén P, Zetterqvist L, Heimdahl A. Long-term results of amalgam versus glass ionomer cement as apical sealant after apicectomy. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1995. 79101–103.
44. Torabinejad M, Rastegar AF, Kettering JD, Pitt Ford TR. Bacterial leakage of mineral trioxide aggregate as a root-end filling material. J Endod 1995. 21109–112.
45. Kettering JD, Torabinejad M. Investigation of mutagenicity of mineral trioxide aggregate and other commonly used root-end filling materials. J Endod 1995. 21537–542.
46. Torabinejad M, Hong CU, Lee SJ, Monsef M, Pitt Ford TR. Investigation of mineral trioxide aggregate for root-end filling in dogs. J Endod 1995. 21603–608.
47. Torabinejad M, Pitt Ford TR, McKendry DJ, Abedi HR, Miller DA, Kariyawasam SP. Histologic assessment of mineral trioxide aggregate as a root-end filling in monkeys. J Endod 1997. 23225–228.
48. Torabinejad M, Hong CU, Pitt Ford TR, Kariyawasam SP. Tissue reaction to implanted SuperEBA and mineral trioxide aggregate in the mandible of guinea pigs: a preliminary report. J Endod 1995. 21569–571.
49. Torabinejad M, Pitt Ford TR, Abedi HR, Tang HM. Tibia and mandible reaction to implanted root-end filling material. J Endod 1997. 23263.
50. Koh ET, Pitt Ford TR, McDonald F. Mineral trioxide aggregate stimulates cytokine production in human osteoblasts. J Bone Miner Res 1995. 10SS406.
51. Koh ET, McDonald F, Pitt Ford Ford, Torabinejad M. Cellular response to Mineral trioxide aggregate. J Endod 1998. 24543–547.

Article information Continued

Copyright © 2002 Korean Academy of Conservative Dentistry
(open-access) :

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Fig. 1

Fig. 1

Schematic diagram of fluid transport device

Fig. 2

Fig. 2

Percentage of Gross leakage (F > 20)

Fig. 3

Fig. 3

A SEM photograph of Am (×3,000)

Fig. 4

Fig. 4

A SEM photograph of GI (×3,000)

Fig. 5

Fig. 5

A SEM photograph of IRM (×3,000)

Fig. 6

Fig. 6

A SEM photograph of MTA (×3,000)

Table 1

Classification of groups

Table 1

Table 2

Result of fluid transport (F:µl/day)

Table 2

Table 3

Multiple comparison by Mann-Whitney rank sum test

Table 3