Antibiotic susceptibility in mutans streptococci and Streptococcus anginosus isolated from dental plaque

Article information

Restor Dent Endod. 2004;29(5):462-469

Publication date (electronic) : 2004 September 30

doi : https://doi.org/10.5395/JKACD.2004.29.5.462

Joong-Ki Kook ¹^,³, Sang-Soo Lim ²^,³, So Young Yoo ¹^,³, Ho-Keel Hwang ²^,³

¹Department of Oral Biochemistry, College of Dentistry, Chosun University, Korea.

²Department of Conservative Dentistry, College of Dentistry, Chosun University, Korea.

³Oral Biology Research Institute, College of Dentistry, Chosun University, Korea.

Corresponding author: Ho-Keel Hwang. Department of Conservative Dentistry, and Oral Biology Research Institute, College of Dentistry, Chosun University 375 Seo-suk Dong, Dong-Gu, Gwang-ju, Korea, 501-749. Tel: 82-62-220-3846, Fax: 82-62-232-9064, rootcanal@hanmail.net

Abstract

The aim of this study was to investigate the susceptibility of mutans streptococci (S. mutans and S. sobrinus) and Streptococcus anginosus, for seven antibiotics, penicillin G, amoxicillin, ciprofloxacin, cefuroxime, erythromycin, bacitracin, and vancomycin. The minimum inhibitory concentration (MIC) of seven antibiotics against 3 species (type strains) of mutans streptococci and S. anginosus, 10 strains (wild type) of S. mutans, 7 strains (wild type) of S. sobrinus, and 11 strains (wild type) of S. anginosus, were measured by broth dilution method. All of the type strains of mutans streptococci and S. anginosus had the same susceptibility for penicillin G, amoxicillin, cefuroxime and bacitracin. Type strain of S. anginosus was sensitive in ciprofloxacin, but those of mutans streptococci were not. All of the clinical isolates of mutans streptococci and S. anginosus had the same susceptibility for the seven antibiotics. Our data reveal that mutans streptococci and S. anginosus have similar antibiotic-resistant character. In addition, these results may offer the basic data to verify the antibiotic-resistant mechanism of mutans streptococci and S. anginosus.

Keywords: Antibiotics; Mutans streptococci; S. anginosus; Susceptibility

References

1. Ruoff KL, Whiley RA, Beighton D. In : Murray ER, Baron EJ, Pfaller MA, Tenover FC, Yolken RH, eds. Streptococcus. Manual of CLINICAL MICROBIOLOGY 1999. 7th Edth ed. Washington: ASM press; 283–296.

2. Whiley RA, Beighton D. Current classification of the oral streptococci. Oral Microbiol Immunol 1998. 13195–216.

3. Igarashi T, Ichikawa K, Yamamoto A, Goto N. Identification of mutans streptococcal species by the PCR products of the dex genes. J Microbiol Methods 2001. 4699–105.

4. Gold OG, Jordan HV, van Houte J. A selective medium for the isolation of Streptococcus mutans. Arch Oral Biol 1973. 181357–1364.

5. Little WA, Korts DC, Thomson LA, Bowen WH. Comparative recovery of Streptococcus mutans on ten isolation media. J Clin Microbiol 1977. 5(6)578–583.

6. Tanzer JM, Borjesson AC, Laskowski L, Kurasz AB, Testa M. Glucose-sucrose-potassium tellurite-bacitracin agar, an alternative to mitis salivarius-bacitracin agar for enumeration of Streptococcus mutans. J Clin Microbiol 1984. 20(4)653–659.

7. Kim PS, Hwang HK, Kim HS, Lim SA, Kang HY, Yoo SY, Kook JK. Identification of non-mutans btreptococci growing on Mitis-Salivarius Bacitracin agar medium. J Dent Res 2003. 82(Special issue B)B-351.

8. Shklair IL, Keene HJ. A biochemical scheme for the separation of the five varieties of Streptococcus mutans. Arch Oral Biol 1974. 191079–1081.

9. Shklair IL, Keene HJ. In : Stiles HM, Loesche WJ, O'Brien TC, eds. Biochemical characterization and distribution of Streptococcus mutans in three diverse populations. Microbial aspects of dental caries 1976. Washington, D.C.: Information Retrieval Inc; 201–210.

10. Murray PR, Jorgensen JH. Quantitative susceptibility test methods in major united states medical center. Antimicrob Agents Chemother 1981. 20(1)66–70.

11. Jorgensen JH, Turnidge JD, Washington A. In : Murray ER, Baron EJ, Pfaller MA, Tenover FC, Yolken RH, eds. Antibacterial susceptibility tests: dilution and disk diffusion methods. Manual of CLINICAL MICROBIOLOGY 1999. 7th Edth ed. Washington: ASM press; 1526–1543.

12. Băncescu G, Skaug N, Dumitriu S, Băncescu A. Antimicrobial susceptibility of some streptococci strains of anginosus group isolated from oral and maxillofacial infections. Roum Arch Microbiol Immunol 1999. 58(1)57–63.

13. Horii T, Arakawa Y, Ohta M, Ichiyama S, Wacharotayankun R, Kato N. Plasmid-mediated AmpC-type beta-lactamase isolated from Klebsiella pneumoniae confers resistance to broad-spectrum beta-lactams, including moxalactam. Antimicrob Agents Chemother 1993. 37(5)984–990.

14. Cloeckaert A, Baucheron S, Flaujac G, Schwarz S, Kehrenberg C, Martel JL, Chaslus-Dancla E. Plasmid-mediated florfenicol resistance encoded by the floR gene in Escherichia coli isolated from cattle. Antimicrob Agents Chemother 2000. 44(10)2858–2860.

15. Simjee S, White DG, McDermott PF, Wagner DD, Zervos MJ, Donabedian SM, English LL, Hayes JR, Walker RD. Characterization of Tn1546 in vancomycin-resistant Enterococcus faecium isolated from canine urinary tract infections: evidence of gene exchange between human and animal enterococci. J Clin Microbiol 2002. 40(12)4659–4665.

16. Bauernfeind A, Wagner S, Jungwirth R, Schneider I, Meyer D. A novel class C beta-lactamase(FOX-2) in Escherichia coli conferring resistance to cephamycins. Antimicrob Agents Chemother 1997. 41(9)2041–2046.

17. Nelson EC, Elisha BG. Molecular basis of AmpC hyperproduction in clinical isolates of Escherichia coli. Antimicrob Agents Chemother 1999. 43(4)957–959.

18. Bonnet R, Chanal C, Ageron E, Sirot D, De Champs C, Grimont P, Sirot J. Inducible AmpC beta-lactamase of a new member Enterobacteriaceae. Antimicrob Agents Chemother 2002. 46(10)3316–3319.

19. Petrosino JF, Pendleton AR, Weiner JH, Rosenberg SM. Chromosomal system for studying AmpC-mediated beta-lactam resistance mutation in Escherichia coli. Antimicrob Agents Chemother 2002. 46(5)1535–1539.

20. Noguchi N, Emura A, Matsuyama H, O'Hara K, Sasatsu M, Kono M. Nucleotide sequence and characterization of erythromycin resistance determinant that encodes macrolide 2'-phosphotransferase I in Escherichia coli. Antimicrob Agents Chemother 1995. 39(10)2359–2363.

21. van Boxtel RA, van de Klundert JA. Expression of the Pseudomonas aeruginosa gentamicin resistance gene aacC3 in Escherichia coli. Antimicrob Agents Chemother 1998. 42(12)3173–3178.

22. Gotoh N, Tsujimoto H, Poole K, Yamagishi J, Nishino T. The outer membrane protein OprM of Pseudomonas aeruginosa is encoded by oprK of the mexA-mexB-oprK multidrug resistance operon. Antimicrob Agents Chemother 1995. 39(11)2567–2569.

23. Mitchell BA, Brown MH, Skurray RA. QacA multidrug efflux pump from Staphylococcus aureus: comparative analysis of resistance to diamidines, biguanidines, and guanylhydrazones. Antimicrob Agents Chemother 1998. 42(2)475–477.

24. Kaatz GW, Seo SM, O'Brien L, Wahiduzzaman M, Foster TJ. Evidence for the existence of a multidrug efflux transporter distinct from NorA in Staphylococcus aureus. Antimicrob Agents Chemother 2000. 44(5)1404–1406.

25. Siqueira JF, Rocas IN, Moraes SR, Santos KR. Direct amplification of rRNA gene sequences for identification of selected oral pathogens in root canal infections. Int Endod J 2002. 35(4)345–351.

26. Tateda M, Shiga K, Saijo S, Sone M, Hori T, Yokoyama J, Matsuura K, Takasaka T, Miyagi T. Streptococcus anginosus in head and neck squamous cell carcinoma: implication in carcinogenesis. Int J Mol Med 2000. 6(6)699–703.

27. Shiga K, Tateda M, Saijo S, Hori T, Sato I, Tateno H, Matsuura K, Takasaka T, Miyagi T. Presence of Streptococcus infection in extra-oropharyngeal head and neck squamous cell carcinoma and its implication in carcinogenesis. Oncol Rep 2001. 8(2)245–248.

28. Kim MS, Kim SG, Chung HM, Kim SG, Kook JK, Kim MK, Kim HS, Yoo SY. Molecular identification of bacteria from osteomyelitis of the jaws. J Korean Assoc Oral Maxillofac Surg 2003. 29(1)48–55.

Article information Continued

(open-access) :

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.